Fig. 3
From: CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells
CCAT1 knockout cells lose the capacity for anchorage-independent growth. a Representative images of soft agar colony formation assay for wild-type and CCAT1 KO cell lines and evaluation of colony count and colony area in these lines. b MTT assay in HT-29, HCT-116, and SW-480 wild-type and CCAT1 KO cell lines performed 2 h and 24 h after seeding. The statistical differences between the wild-type and knockout cell lines are analyzed by Mann-Whitney test. The scale bars in panel A denote 200 μm