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Table 1 Comparison of the features of anti-HBs qELISA_v.1 and anti-HBs qELISA_v.2

From: A New ELISA to Overcome the Pitfalls in Quantification of Recombinant Human Monoclonal Anti-HBs, GC1102, by Commercial Immunoassays

  Anti-HBs qELISA_v.1 Anti-HBs qELISA_v.2
Sample dilution buffer 1% BSA in PBS, pH 7.2 10% BSA, 0.1% skim milk and 0.05% Tween 20 in PBS, pH 7.2
Secondary antibody dilution buffer 1% BSA in PBS, pH 7.2 10% Goat Serum in PBS, pH 7.2
Washing buffer PBS-T (PBS, pH 7.2, containing 0.05% Tween 20) 20 mM sodium acetate buffer, pH 4.0, containing 150 mM sodium chloride and 0.05% Tween 20