A New ELISA to Overcome the Pitfalls in Quantification of Recombinant Human Monoclonal Anti-HBs, GC1102, by Commercial Immunoassays

Table 1 Comparison of the features of anti-HBs qELISA_v.1 and anti-HBs qELISA_v.2

	Anti-HBs qELISA_v.1	Anti-HBs qELISA_v.2
Sample dilution buffer	1% BSA in PBS, pH 7.2	10% BSA, 0.1% skim milk and 0.05% Tween 20 in PBS, pH 7.2
Secondary antibody dilution buffer	1% BSA in PBS, pH 7.2	10% Goat Serum in PBS, pH 7.2
Washing buffer	PBS-T (PBS, pH 7.2, containing 0.05% Tween 20)	20 mM sodium acetate buffer, pH 4.0, containing 150 mM sodium chloride and 0.05% Tween 20

ISSN: 1480-9222