Fig. 3

Schematic illustration for measuring anti-HBs titers by ELISA. Blue colored Y denotes GC1102 and green colored Y denotes goat anti-human IgG. “E” denotes an enzyme in this ELISA. a General principle of sandwich ELISA for measuring anti-HBs. Anti-HBs in the sample forms a bridge between the immobilized HBsAg and the labeled HBsAg, and signals produced thereby are measured. b Possibility of not forming the bridge due to a masking of antibody binding sites by immobilized and/or labeled HBsAg. c Indirect ELISA for correct quantification of anti-HBs. Any anti-HBs bound to the immobilized HBsAg can be detected by HRP labeled goat anti-human IgG