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Fig. 4 | Biological Procedures Online

Fig. 4

From: Targeting of HER3 with Functional Cooperative miRNAs Enhances Therapeutic Activity in HER2-Overexpressing Breast Cancer Cells

Fig. 4

Concurrent expression of miR-125a and miR-205 via transfection with the miRNA cluster significantly inhibited proliferation, induced cell cycle G1 arrest, and inactivated HER3 signaling in BT474 cells. a The stable sub-clones of BT474 cells transfected with empty vector (pCDH), pCDH-miR-125a, or pCDH-miR-205 alone or pCDH-miR-125a-miR-205 (cluster) were selected. The relative expression levels of miR-125a and miR-205 were measured by qRT-PCR using TaqMan miRNA assays. In the cluster cell clone, both miR-125a and miR-205 were upregulated. b Proliferation of each cell clone was examined via analysis of the IncuCyte system. Bars, SD. Data shows the representative of three independent experiments. c Cell cycle progression was analyzed by flow cytometry. d The stable sub-clones were collected and subjected to western blot analyses with the specific antibodies directed against p-erbB3, HER3 (erbB3), p-Src, Src, p-Akt, Akt, p-MAPK, MAPK, or β-actin

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