Fig. 3

Co-expression of miR-125a and miR-205 via transient transfection with the multi-miRNA expression vector (cluster) exhibited potent activity to inhibit erbB3 expression in HER2-overexpressing breast cancer cells. a Map of the multi-miRNA lentivector expression vector (cluster). The precursors of miR-125a and miR-205 were linked together and driven by the same CMV promoter. b SKBR3 cells and BT474 cells were infected with the lentivirus containing pCDH-miR-125a, pCDH-miR-125b, or pCDH-miR-205 alone or the pCDH-miR-125a-miR-205 (cluster) for 48 h. The expression levels of miR-125a, miR-125b, and miR-205 were measured by qRT-PCR using TaqMan miRNA assays. All results were normalized with the internal control RNU6B. Bars, SD. Data shows the representative of three independent experiments. The expression levels of HER3 (erbB3) were detected by western blot analysis upon lentiviral infection for 72 h. β-actin was used as a loading control