Fig. 2

Ectopic expression of miR-125a, miR-125b, and/or miR-205 via transient transfection inhibited erbB3 expression in HER2-overexpressing breast cancer cells. BT474 cells were infected with the lentivirus containing pCDH-miR-125a, pCDH-miR-125b, or pCDH-miR-205 alone or both pCDH-miR-125a and pCDH-miR-205 or both pCDH-miR-125b and pCDH-miR-205 for 48 h (a) or 72 h (b). The expression levels of miR-125a, miR-125b, and miR-205 were measured by qRT-PCR using TaqMan miRNA assays. All results were normalized with the internal control RNU6B. Bars, SD. Data shows the representative of three independent experiments. c The expression levels of HER3 (erbB3) were detected by western blot analysis upon lentiviral infection for 72 h. β-actin was used as a loading control