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Fig. 2 | Biological Procedures Online

Fig. 2

From: Real-Time Vital Mineralization Detection and Quantification during In Vitro Osteoblast Differentiation

Fig. 2

Mineralization dynamics of differentiating osteoblasts treated with osteogenic cocktail (OGC), detected by Calcein. a Fluorescent images of the cell culture obtained by IncuCyte ZOOM at day 7, day 10 and day 14 of differentiation. Last row: vehicle (ethanol) treated cells, showing no differentiation, representative pictures are shown, n = 3. b Mineralization curve generated automatically by the IncuCyte ZOOM based on the green object count per image. The cells were scanned automatically every 3 h for two weeks, representative experiment is shown, n = 3, SE

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