Fig. 6
From: Optimization of Immunofluorescent Detection of Bone Marrow Disseminated Tumor Cells
The optimized protocol enhances detection capability of automated software as compared to the basic protocol. Comparison of the number of pan cytokeratin+ and white blood cell+ (CK + WBC+) cells detected within a defined area with automated scanning software using the basic and optimized protocols for primary stained, cancer cell-spiked slides from 5A. After the software generated a gallery of green-positive cells (CK+ candidates; total populated), the gallery was manually scanned to eliminate green-negative events (background signal only due to high exposure time; first selection) and then a second selection was applied to eliminate debris and non-cellular events. This left a gallery with candidate cancer cells (CK + WBC-) and double positive WBCs with false CK signal (CK + WBC+)