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Table 1 Recipes for Buffers used for the Protocol

From: Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin

Components Volume
50X TAE Buffer (500 ml)  
Tris Buffer GR 121 g
EDTA Stock (0.5 M) 50 ml
Glacial Acetic Acid 28.55 ml
5× TBE Buffer (1 l, pH 8.3)  
Tris Buffer GR 54 g
Boric Acid 27.5 g
EDTA Stock (0.5 M) 20 ml
30% Acrylamide (25 ml)  
Acrylamide 7.3 g
Bis-acrylamide 0.2 g
8% Polyacrylamide Gel  
30% Acrylamide 2.620 ml
5x TBE Buffer 1.97 ml
Autoclaved Milli Q water 5.24 ml
10% Ammonium Persulfate 163 μl
TEMED 8 μl
Total 10 ml
EtBr (for staining) 0.5 μg/ml in 1X TBE
10X TE Buffer (pH 8.0)  
Tris Buffer 1 ml (Stock 1 M; Final concentration 100 mM)
EDTA 200 μl (Stock 1 M; Final concentration 10 mM)
Autoclaved Milli Q water 8.8 ml