Fig. 3

Comparison between PCR products obtained from zebra finch blood samples using the old and modified Chelex method. a Clear bands were obtained using the old (left) and modified (right) protocols to analyse the product from Z1/Z2 W1/W2 primer pairs in zebra finch blood samples from adult male birds. The bands represent PCR products obtained from two, one, half and one fourth of a Whatman filter paper disc, beginning at the lane closest to the marker and moving outwards towards the edge of the gel, respectively. b The second gel shows no bands obtained by using the old protocol (left), whereas clear bands were obtained by using the modified protocol (right). Band representation remains the same