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Table 2 Primers sequences and names were developed for isolation and gene expression of SOS1, NHX in K.scoparia

From: Isolation and Semi Quantitative PCR of Na+/H+ Antiporter (SOS1 and NHX) Genes under Salinity Stress in Kochia scoparia

Gene Primers Name Primers Sequences application
SOS1 P.SOS.S.FWD1 P.SOS.S.FWD2 P.SOS.S.FWD3
P.SOS.S.FWD4
P.SOS.S.REV1 P.SOS.S.REV2
P.SOS.S.REV3
P.SOS.S.REV4
P.SOS.S.FWD1
5- ATG GCA GCA TCT CGA A-3
5- ACT GGA ACA CTG TTT-3
5- CAA ATG GGA TCT GGC T-3
5- CAC TTT TGG GAG ATG GT-3
5-AGA AAA CAA ACA ATG TTC C -3
5- CCG TTT GAT ATA AGC CA-3
5- GAG ATT ACT TGG TGA ATC-3
5- CAA TAA CAC TTT CCT TCC A-3
5- ATG GCA GCA TCT CGA A-3
 
NHX P.NHX.S.FWD1
P.NHX.S.REV1
P.NHX.S.FWD2
P.NHX.S.REV2
5-ATGTGGTCACAGTTAAGC-3
5-ATAAGCCATAAGCATCAT − 3
5-GTGAGGTTGCTTTAATG-3
5-CCAAATACAGGCCGCAT-3
Gene sequencing
  P.NHX.S.FWD3
P.NHX.S.REV3
P.NHX.S.FWD1
P.NHX.S.REV1
5-ACAGATTCTGTTTGCAC-3
5-CATAAGACCAGCCCACCA-3
5-ATGTGGTCACAGTTAAGC-3
5-ATAAGCCATAAGCATCAT − 3
 
SOS1 P.SOS.G.F P.SOS.G.R 5-GGAAGGTTTGGGGATGGTAT-3
5-GTCCAGCAAGCAAACCATT-3
Gene expression
NHX P.NHX.G.F P.NHX.G.R 5-TTCTGGATTGCTCAGTGCTT-3
5-CAGCCAGCATGTAAGAGAGG-3
Gene expression
18srRNA Forward
Reverse
5-ATGATAACTCGACGGATCGC-3 5-CTTGGATGTGGTAGCCGTTT-3 Gene expression