Table 2 Primers sequences and names were developed for isolation and gene expression of SOS1, NHX in K.scoparia
Gene | Primers Name | Primers Sequences | application |
|---|---|---|---|
SOS1 | P.SOS.S.FWD1 P.SOS.S.FWD2 P.SOS.S.FWD3 P.SOS.S.FWD4 P.SOS.S.REV1 P.SOS.S.REV2 P.SOS.S.REV3 P.SOS.S.REV4 P.SOS.S.FWD1 | 5- ATG GCA GCA TCT CGA A-3 5- ACT GGA ACA CTG TTT-3 5- CAA ATG GGA TCT GGC T-3 5- CAC TTT TGG GAG ATG GT-3 5-AGA AAA CAA ACA ATG TTC C -3 5- CCG TTT GAT ATA AGC CA-3 5- GAG ATT ACT TGG TGA ATC-3 5- CAA TAA CAC TTT CCT TCC A-3 5- ATG GCA GCA TCT CGA A-3 | Â |
NHX | P.NHX.S.FWD1 P.NHX.S.REV1 P.NHX.S.FWD2 P.NHX.S.REV2 | 5-ATGTGGTCACAGTTAAGC-3 5-ATAAGCCATAAGCATCAT − 3 5-GTGAGGTTGCTTTAATG-3 5-CCAAATACAGGCCGCAT-3 | Gene sequencing |
|  | P.NHX.S.FWD3 P.NHX.S.REV3 P.NHX.S.FWD1 P.NHX.S.REV1 | 5-ACAGATTCTGTTTGCAC-3 5-CATAAGACCAGCCCACCA-3 5-ATGTGGTCACAGTTAAGC-3 5-ATAAGCCATAAGCATCAT − 3 |  |
SOS1 | P.SOS.G.F P.SOS.G.R | 5-GGAAGGTTTGGGGATGGTAT-3 5-GTCCAGCAAGCAAACCATT-3 | Gene expression |
NHX | P.NHX.G.F P.NHX.G.R | 5-TTCTGGATTGCTCAGTGCTT-3 5-CAGCCAGCATGTAAGAGAGG-3 | Gene expression |
18srRNA | Forward Reverse | 5-ATGATAACTCGACGGATCGC-3 5-CTTGGATGTGGTAGCCGTTT-3 | Gene expression |