Fig. 4
Hfq complexes smelA075 in M. truncatula nodules. a RT-PCR detected smelA075 from Hfq complexes immunoprecipitated from lysates of M. truncatula nodules 10-dpi (lane 2), 18-dpi (lane 3) and 49-dpi (lanes 4), but failed to detect smelA075 from control samples (lane 5). Markers (lane 1). This experiment was repeated twice and similar results were obtained (data not shown). b smelA075 immunoprecipitated with the HfqHis was analyzed by qPCR. Relative enrichments were calculated with the 2-∆∆Ct method [39]. The data are presented as mean fold change ± SEM and normalized to S. meliloti 16S rRNA gene