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Fig. 3 | Biological Procedures Online

Fig. 3

From: DNA Area and NETosis Analysis (DANA): a High-Throughput Method to Quantify Neutrophil Extracellular Traps in Fluorescent Microscope Images

Fig. 3

Detection of DAPI-stained murine NETs by DANA. Murine neutrophils were either left untreated (UT) or stimulated (STIM) for 4 h to induce NETosis on coverslips, then fixed, stained with DAPI, mounted, and imaged at 400×. a. Two individuals independently counted the number of nuclei and NET-like structures by eye and then DANA was used to calculate the percent NETosis for all images (n = 12 images). The mean absolute difference with SEM for percent NETosis per image was graphed for DANA versus individual 2 by eye, DANA versus individual 3 by eye, and individual 2 versus individual 3 by eye. DANA was used to quantify percent NETosis (b) and DNA area (c) with average and SEM graphed for unstimulated and stimulated murine neutrophils (n = 5 mice). For all panels, **p < 0.01

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