Fig. 2

Single in situ hybridization of Pou5f1/Oct4 mRNA (purple) in mouse ovaries. a-b Mouse ovary sections hybridized with the antisense (a) or sense (b) Pou5f1/Oct4 RNA probe without amplification of signals using the TSA system (-TSA). No signal was detected in this conventional in situ hybridization method. c-d Mouse ovary sections hybridized with the antisense (c) or sense (d) Pou5f1/Oct4 RNA probe with amplification of signals using the TSA system (+TSA). The signals were detected in oocytes of sections hybridized with the antisense RNA probe (c) but not in those hybridized with the sense RNA probe (d). Arrows indicate oocytes observed in the sections. Bars: 100 μm