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Table 1 Fixative combinations used in this study. All fixative combinations were performed in triplicate on 16HBE14o- cultured cells. Immunocytochemistry was performed on cells as detailed

From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial Cells

Pre-extraction Fixation Permeabilization
4% Paraformaldehyde
0.2% Triton X-100 4% Paraformaldehyde
4% Paraformaldehyde 0.1% Saponin
4% Paraformaldehyde + Acetone
100% Methanol
0.2% Triton X-100 100% Methanol
100% Methanol 0.1% Saponin
1:1 Methanol:Acetone
100% Acetone