Table 1 Fixative combinations used in this study. All fixative combinations were performed in triplicate on 16HBE14o- cultured cells. Immunocytochemistry was performed on cells as detailed
From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial Cells
Pre-extraction | Fixation | Permeabilization |
|---|---|---|
– | 4% Paraformaldehyde | – |
0.2% Triton X-100 | 4% Paraformaldehyde | – |
– | 4% Paraformaldehyde | 0.1% Saponin |
– | 4% Paraformaldehyde + Acetone | – |
– | 100% Methanol | – |
0.2% Triton X-100 | 100% Methanol | – |
– | 100% Methanol | 0.1% Saponin |
– | 1:1 Methanol:Acetone | – |
– | 100% Acetone | – |