Fig. 4From: Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial CellsMethanol-acetone fixation of primary airway epithelial cells (AEC) grown at air liquid interface (ALI). The top row of panels show positive staining for both claudin-1 and ZO-1. The bottom row of panels show positive co-staining of occludin and ZO-1 in primary AECs grown at ALI. Merged images showed nuclei staining with Hoechst (blue)Back to article page