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Table 2 MoDCs’ differentiation: efficiency, viability, maturation and co-stimulation markers

From: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

 

MACS

EasySep

Differentiation efficiency (%)a

44.57 ± 3.63

35.85 ± 3.21 b

7AAD (%)

14.04 ± 3.15

22.17 ± 3.75 b

HLA-DR (MFI)

720.0 ± 100.0

995.8 ± 178.8

  1. Values represent Mean ± SEM values from 5 different donors
  2. aDifferentiation efficiency was calculated based on the percentage of monocytes that differentiated into MoDCs, calculated by dividing the number of viable MoDCs collected by the number of monocytes that had been initially plated
  3. bMarginal significance (0.05 < p < 0.1) refers to differences between monocyte isolation using both methods
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Biological Procedures Online

ISSN: 1480-9222

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