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Fig. 4 | Biological Procedures Online

Fig. 4

From: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

Fig. 4

Comparison of cytokine secretion by T cells when co-cultured with tumor antigen-loaded MoDCs. T cells were cultured with autologous MoDCs (1 MoDC: 5 T cell) loaded or not with whole tumor cell lysates (TL) from MCF-7 breast cancer cell line. Control conditions with unstimulated T cells were run in parallel. After 3 to 5 days in co-culture, cytokine expression was evaluated by ELISA technique: (a) IFN-γ expression; (b) TNF-α expression. Black bars represent data obtained from T cells stimulated with MACS_MoDCs and grey bars data related to EasySep_MoDCs. Data refers to the mean cytokine secretion (pg/mL) ± SEM from 3 to 4 different donors. Asterisks represent statistical significance (*p < 0.05; **p < 0.01)

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