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Fig. 3 | Biological Procedures Online

Fig. 3

From: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

Fig. 3

Comparison of activation level between MoDCs and responsiveness to maturation stimuli or tumor antigens. MACS_MoDCs (black bars, MACS) and EasySep_MoDCs (grey bars, EasySep) were loaded with tumor cell lysates (TL) from MCF-7 breast cancer cell line or stimulated with LPS and TNF-α during 4 h and analyzed by flow cytometry for the expression of (a) HLA-DR, and (b) CD86. Unstimulated MoDCs were used as controls. Data shown refer to mean ± SEM of MFI (n = 3 different donors)

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