Fig. 2

Comparison of MoDCs viability and maturation. After isolating monocytes with MACS or EasySep technologies, cells were cultured with GM-CSF and IL-4 during 1 week to allow their differentiation into MoDCs, as described in the Material and Methods section. MoDCs were collected and stained with different makers: 7AAD to assess cell viability (a, b); HLA-DR, a maturation marker (c, d); CD86, a co-stimulatory molecule (e, f). a, c, e– MoDCs derived from monocytes isolated with MACS technology; b, d, f– MoDCs derived from monocytes isolated with EasySep technology. Cells were analyzed by flow cytometry and the presented histograms refer to the population of stained MoDC (dark grey) and unstained control (light grey). A representative histogram of one out of eight different donors is shown