Fig. 3
From: Gibson Deletion: a novel application of isothermal in vitro recombination
Use of Gibson Deletion to change, delete or maintain a restriction site. a Schematic depiction of the two groups of changes introduced in pUC19 using Gibson Deletion: top = making PvuII site unique changing a site into an AflII site and maintain the other PvuII site; bottom = making NspI site unique deleting a site, maintaining a second site and changing a third NspI site into an AflII site. b Results from the cloning depicted in a