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Table 1 Quality and quantity of extracted RNA from FACS purified hLESCs subpopulations

From: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

 

Profile

Cell numbers

RIN

RNA yield (pg/cell)

SP1

ABCB5+p63+CK3+

345 × 103

8.1

6.37

SP2

ABCB5+p63+CK3−

94 × 103

7.5

4.97

SP3

ABCB5+p63−CK3−

73 × 103

7.3

4.75

SP4

ABCB5−p63+CK3−

109 × 103

7.9

2.25

  1. FACS-sorted hLESCs subpopulations from three independent experiments were pooled for RNA extaction and subsequent evaluation of RNA integrity and quantity. RIN = RNA integrity number; SP = subpopulation
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Biological Procedures Online

ISSN: 1480-9222

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