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Table 1 Quality and quantity of extracted RNA from FACS purified hLESCs subpopulations

From: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

  Profile Cell numbers RIN RNA yield (pg/cell)
SP1 ABCB5+p63+CK3+ 345 × 103 8.1 6.37
SP2 ABCB5+p63+CK3 94 × 103 7.5 4.97
SP3 ABCB5+p63CK3 73 × 103 7.3 4.75
SP4 ABCB5p63+CK3 109 × 103 7.9 2.25
  1. FACS-sorted hLESCs subpopulations from three independent experiments were pooled for RNA extaction and subsequent evaluation of RNA integrity and quantity. RIN = RNA integrity number; SP = subpopulation