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Fig. 2 | Biological Procedures Online

Fig. 2

From: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

Fig. 2

Effect of optimized procedure on the separation of subpopulations and the sequencing analysis of RNA quality. a Discrimination from background using top 2.5 percentile of control intensity as a cut-off is shown in representative traces for both membrane (ABCB5) and intracellular (p63 and CK3) antigens. Robust and specific sorting produced four subpopulations indicated in boldface. b The quality of reads was assessed by sequence quality (PHRED score), mapping coverage, and GC-content distribution

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