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Fig. 2 | Biological Procedures Online

Fig. 2

From: Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of Proteins

Fig. 2

Examination of the efficiency of DpnI digestion of the PCR products. The PCR products of plasmid pET42a-NAP encoding the napA gene with the indicated mutations, E97GY101H, D98A, Y99A, Y101H, and E103D, and no-primer PCR control (NPC) were left untreated (−) or treated (+) with restriction enzyme DpnI and then analyzed by 1% agarose gel electrophoresis. Arrows indicate the PCR products resistant to the DpnI digestion. Migration of supercoiled (sc), nicked circular (nc), circular single-stranded (css), and primer-dimer (pd) DNA is indicated. Lane M: 1 kb DNA ladder (SMOBiO, Hsinchu, Taiwan)

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