Fig. 2From: Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of ProteinsExamination of the efficiency of DpnI digestion of the PCR products. The PCR products of plasmid pET42a-NAP encoding the napA gene with the indicated mutations, E97GY101H, D98A, Y99A, Y101H, and E103D, and no-primer PCR control (NPC) were left untreated (−) or treated (+) with restriction enzyme DpnI and then analyzed by 1% agarose gel electrophoresis. Arrows indicate the PCR products resistant to the DpnI digestion. Migration of supercoiled (sc), nicked circular (nc), circular single-stranded (css), and primer-dimer (pd) DNA is indicated. Lane M: 1 kb DNA ladder (SMOBiO, Hsinchu, Taiwan)Back to article page