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Table 2 Effects of SV40LT and H-RASV12 genes in porcine blastocyst development of SCNT embryos

From: SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

Donor cells No. of Fused (%) No. (%)* of embryos developed to
oocytes ≥2-cell Blastocyst
Con-Pig cells 250 169 (71.8 ± 6.1)a b 124 (73.8 ± 4.0)a 33 (19.5 ± 1.2)a
SV40LT-Pig cells 246 141 (61.0 ± 4.7)a 69 (48.6 ± 2.4)b 8 (5.6 ± 1.8)b
SV40LT + HrasV12-Pig cells 274 201 (82.0 ± 4.7)b 153 (72.8 ± 8.2)a 13 (6.2 ± 1.8)b
  1. The cleavage rate (2–8-cell stage) was measured on day 2 and the blastocyst formation rate was evaluated on day 7 of culture
  2. Values with different superscript letters (a, b, c) within a column differ significantly (p < 0.05)
  3. Experiment was repeated three times
  4. The data represent the means ± SEM
  5. *Percentage of total fused oocytes