SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

Table 2 Effects of SV40LT and H-RAS^V12 genes in porcine blastocyst development of SCNT embryos

Donor cells	No. of	Fused (%)	No. (%)* of embryos developed to
Donor cells	oocytes	Fused (%)	≥2-cell	Blastocyst
Con-Pig cells	250	169 (71.8 ± 6.1)^{a b}	124 (73.8 ± 4.0)^a	33 (19.5 ± 1.2)^a
SV40LT-Pig cells	246	141 (61.0 ± 4.7)^a	69 (48.6 ± 2.4)^b	8 (5.6 ± 1.8)^b
SV40LT + HrasV12-Pig cells	274	201 (82.0 ± 4.7)^b	153 (72.8 ± 8.2)^a	13 (6.2 ± 1.8)^b

The cleavage rate (2–8-cell stage) was measured on day 2 and the blastocyst formation rate was evaluated on day 7 of culture
Values with different superscript letters (a, b, c) within a column differ significantly (p < 0.05)
Experiment was repeated three times
The data represent the means ± SEM
*Percentage of total fused oocytes

ISSN: 1480-9222