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Table 1 Pregnancy outcomes of canine embryos derived from SCNT using control and SV40LT-expressing fibroblast cells

From: SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

Donors

Nuclear transfer

Embryo transfer

No. of

No. of surrogate dogs

No. of pregnancies (%)†

Oocyte donor dogs

Oocyte subjected to NT

Oocyte fused & transferred (%)*

Sac observed (%)**

Offspring born (%)**

On Day 30

Full Term‡

Con-K9 cells

14

121

109 (90.1)a

3 (2.8)a

3 (2.8)a

7

3 (21.1)a

3 (21.1)a

SV40LT-K9 cells

72

696

305 (43.8)

0 (0.0)

0 (0.0)

21

0 (0.0)

0 (0.0)

  1. †Pregnancy was detected by real-time ultrasonography. Pregnancy rate was calculated based on the number of pregnancies observed of the total number of surrogates transferred with reconstructed embryos (fused oocytes)
  2. ‡Full term pregnancy refers to pregnancy that successfully produces living offspring
  3. *Fusion rate was calculated based on the number of fused oocytes of the total oocytes subjected to nuclear transfer
  4. **The rate of sac observed and offspring born was calculated based on the total number of reconstructed embryos transferred
  5. Values with superscript letter (a) within a column differ significantly (p < 0.05)
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