Fig. 4
SV40LT did not transform porcine fetal fibroblast cells. a Western blotting analysis showing expression of SV40LT or H-RASV12 in control porcine fetal fibroblast cells (Cloud male #5), cells expressing SV40LT, and cells expressing SV40LT and H-RASV12. β-Actin was used as a loading control. b Microscopic images showing cellular morphology of control porcine fetal fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RASV12. Scale bars indicate 10 μm. c Microscopic images showing colonies formed under soft agar culture conditions (left photos). Quantitative analysis showing colony number of control porcine fetal fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RASV12 (right graph). Scale bars indicate 5 μm