Fig. 2

Non-transformed characteristics of SV40LT-mediated immortalized canine fibroblast cells. a Western blotting analysis showing expression of SV40LT or H-RASv12 in control K9 fetus 1 fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RAS^V12. β-Actin was used as a loading control. b Microscopic images showing cellular morphology of control K9 fetus 1 fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RAS^V12. Scale bars indicate 5 μm. c Microscopic images showing colonies formed under soft agar culture conditions (left photos). Quantitative analysis showing colony number of control K9 fetus 1 fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RAS^V12 (right graph). Scale bars indicate 3 μm. d Representative photos (left panel) showing immuno-deficient mice injected with control K9 fetus 1 fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RAS^V12. Arrows (red) indicate xenograft tumors derived from cells expressing SV40LT and H-RAS^V12. Quantitative analysis showing in vivo tumorigenic potential of control K9 fetus 1 fibroblast cells, cells expressing SV40LT, and cells expressing SV40LT and H-RAS^V12 (right graph)