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Fig. 1 | Biological Procedures Online

Fig. 1

From: SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

Fig. 1

Immortalization of canine primary fibroblast cells via ectopic expression of SV40LT. a Cell growth rates (fold-changes) of different passages of K9 fetus 1 fibroblast cells were examined by counting 3 days after plating (1 × 105). b Western blotting analysis showing expression of SV40LT in control K9 fetus 1 fibroblast cells and cells expressing SV40LT. β-Actin was used as a loading control. c Cumulative growth curves of control K9 fetus 1 fibroblast cells and cells expressing SV40LT. d Microscopic images showing cellular morphology of control K9 fetus 1 fibroblast cells (passages 3 and 13) and cells expressing SV40LT (passage 13 after antibiotic selection). Scale bars indicate 5 μm. e Senescence-associated β-galactosidase (SA-β-gal) stain assay of control (passages 3 and 13) and SV40LT-overexpressing K9 fetus 1 fibroblast cells (passage 13). Arrows indicate SA-β-gal-positive cells in passage 13 of control K9 fetus 1 fibroblast cells. Scale bars indicate 5 μm. f Quantitative analysis of SA-β-gal-positive cells presented in (E). P# indicates passage number of cells

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