Fig. 5

5mC and 5hmC labelling in the control (C), culture-control (CC) and 5-Aza-CdR-treated (Azac1 and AzaC3) gonads of 16dpc embryos. 5mC is detected in C (a) and CC (b) PGC (arrows) and somatic cells (arrowheads). In the gonads treated with 1 μM of 5-Aza-CdR (c) very weak labelling was observed in PGC (arrows) whereas the somatic cells showed more intense labelling (arrowheads). The gonads treated with 3 μM of 5-Aza-CdR (d) showed weak labelling in PGC (arrows) and somatic cells (arrowheads). 5hmC was detected in restricted regions of the germ cell nuclei in control (C) gonads (arrows) and in the whole nucleus of Sertoli cells (arrowheads) (e). In control-culture gonads (CC) germ cells (arrows) show weak labeling in the whole nucleus, whereas in the Sertoli cells (arrowheads) 5hmC labeling did not change when compared with C gonads (f). After 5-Aza-CdR (3μM) treatment (AzaC1), 5hmC labeling was more intense in germ cells (arrows) when compared with the CC gonads, whereas no alteration was observed in Sertoli cells (arrowheads) (g)