Fig. 1

DNMT1, DNMT3a and DNMT3b labelling in male rat embryonic gonads. DNMT1 was not detected in PGC (arrowheads) at 14dpc (a); the somatic cells were positive (arrows). At 15dpc (b) and 19dpc (c) all PGC/gonocytes were positive (arrows). These results coincides with 5mC labelling, indicating that PGC reprogramming ends around 15dpc. DNMT3a was not detected in PGC (arrows) at 14dpc (d) and 15dpc (e). At 19dpc (f) DNMT3a was detected (arrows) in all gonocytes, although the labelling intensity has varied among them. At 14dpc (g) and 15dpc (h) DNMT3b-positive (arrows) and DNMT3b-negative (arrowheads) PGC were observed. At 19dpc (i) DNMT3b was not detected (arrowheads) in the gonocytes and was detected in Sertoli cells (arrows)