Cell Counting and Viability Assessment of 2D and 3D Cell Cultures: Expected Reliability of the Trypan Blue Assay

Piccinini, Filippo; Tesei, Anna; Arienti, Chiara; Bevilacqua, Alessandro

doi:10.1186/s12575-017-0056-3

Biological Procedures Online

Table 1 Original measurements for all S _k analysed by O ₁ and O ₂

From: Cell Counting and Viability Assessment of 2D and 3D Cell Cultures: Expected Reliability of the Trypan Blue Assay

		O ₁			O ₂
		Live cells	Dead cells	Viability [%]	Live cells	Dead cells	Viability [%]
A ₁	S ₁	271	39	87.42	306	33	90.27
	S ₂	330	51	86.61	339	41	89.21
	S ₃	327	37	89.84	297	28	91.38
	S ₄	363	24	93.80	345	23	93.75
	S ₅	336	40	89.36	394	30	92.92
A ₂	S ₁	234	92	71.78	325	77	80.85
	S ₂	178	57	75.74	320	71	81.84
	S ₃	176	48	78.57	274	53	83.79
	S ₄	250	67	78.86	204	55	78.76
	S ₅	442	102	81.25	244	50	82.99
A ₃	S ₁	277	114	70.84	218	79	73.40
	S ₂	259	108	70.57	241	87	73.48
	S ₃	297	111	72.79	309	101	75.37
	S ₄	253	76	76.90	220	182	54.73
	S ₅	247	86	74.17	178	64	73.55
A ₄	S ₁	248	84	74.70	364	137	72.65
	S ₂	326	121	72.93	390	136	74.14
	S ₃	173	53	76.55	407	133	75.37
	S ₄	303	105	74.26	343	119	74.24
	S ₅	301	106	73.96	364	122	74.90
A ₅	S ₁	131	119	52.40	202	145	58.21
	S ₂	130	113	53.50	218	227	48.99
	S ₃	143	64	69.08	110	24	82.09
	S ₄	166	64	72.17	172	49	77.83
	S ₅	166	83	66.67	259	68	79.20
A ₆	S ₁	91	12	88.35	162	88	64.80
	S ₂	46	35	56.79	116	76	60.42
	S ₃	81	33	71.05	83	40	67.48
	S ₄	93	49	65.49	100	48	67.57
	S ₅	101	50	66.89	128	60	68.09
A ₇	S ₁	198	206	49.01	108	103	51.18
	S ₂	244	267	47.75	165	126	56.70
	S ₃	208	163	56.06	249	190	56.72
	S ₄	207	130	61.42	177	146	54.80
	S ₅	146	120	54.89	201	174	53.60
A ₈	S ₁	111	181	38.01	142	200	41.52
	S ₂	147	294	33.33	121	220	35.48
	S ₃	178	179	49.86	199	220	47.49
	S ₄	169	137	55.23	129	142	47.60
	S ₅	147	118	55.47	106	128	45.30
P ₁	S ₁	107	11	95.24	100	5	90.68
	S ₂	80	8	96.25	77	3	90.91
	S ₃	101	9	95.18	79	4	91.82
	S ₄	83	7	95.59	65	3	92.22
	S ₅	70	6	95.65	88	4	92.11
P ₂	S ₁	106	17	86.87	86	13	86.18
	S ₂	118	21	90.00	99	11	84.89
	S ₃	99	12	87.60	106	15	89.19
	S ₄	107	12	80.00	80	20	89.92
	S ₅	119	14	78.50	84	23	89.47
P ₃	S ₁	63	14	77.61	52	15	81.82
	S ₂	46	14	74.14	43	15	76.67
	S ₃	52	10	81.69	58	13	83.87
	S ₄	75	17	72.73	56	21	81.52
	S ₅	52	11	75.86	44	14	82.53
P ₄	S ₁	55	48	54.17	39	33	53.40
	S ₂	57	44	43.48	30	39	56.44
	S ₃	49	44	51.04	49	47	52.69
	S ₄	40	30	55.65	69	55	57.14
	S ₅	38	42	57.43	85	63	47.50
P ₅	S ₁	14	116	11.59	8	61	10.77
	S ₂	13	91	9.26	5	49	12.50
	S ₃	15	127	16.22	12	62	10.56
	S ₄	18	138	10.26	8	70	11.54
	S ₅	11	71	13.33	10	65	13.41
SP ₁	S ₁	100	69	59.17	133	82	61.86
	S ₂	116	106	52.25	94	72	56.63
	S ₃	136	88	60.71	72	39	64.86
	S ₄	116	87	57.14	100	40	71.43
	S ₅	163	96	62.93	80	45	64.00
SP ₂	S ₁	155	120	56.36	66	73	47.48
	S ₂	125	94	57.08	125	71	63.78
	S ₃	158	87	64.49	103	74	58.19
	S ₄	154	75	67.25	85	68	55.56
	S ₅	156	81	65.82	219	177	55.30
SP ₃	S ₁	167	42	79.90	117	18	86.67
	S ₂	191	40	82.68	97	13	88.18
	S ₃	128	41	75.74	180	23	88.67
	S ₄	109	39	73.65	113	21	84.33
	S ₅	146	34	81.11	130	22	85.53
SP ₄	S ₁	101	71	58.72	58	33	63.74
	S ₂	114	65	63.69	163	61	72.77
	S ₃	92	60	60.53	141	45	75.81
	S ₄	92	53	63.45	124	60	67.39
	S ₅	179	77	69.92	121	56	68.36
SP ₅	S ₁	260	96	73.03	140	57	71.07
	S ₂	207	88	70.17	282	45	86.24
	S ₃	232	64	78.38	173	53	76.55
	S ₄	192	56	77.42	209	53	79.77
	S ₅	263	75	77.81	69	24	74.19
SP ₆	S ₁	222	65	77.35	175	41	81.02
	S ₂	226	66	77.40	229	59	79.51
	S ₃	216	53	80.30	108	29	78.83
	S ₄	218	54	80.15	135	37	78.49
	S ₅	205	44	82.33	254	43	85.52
SP ₇	S ₁	134	101	57.02	159	93	63.10
	S ₂	161	128	55.71	235	124	65.46
	S ₃	151	134	52.98	83	70	54.25
	S ₄	180	106	62.94	134	97	58.01
	S ₅	190	119	61.49	91	78	53.85
SP ₈	S ₁	146	197	42.57	67	105	38.95
	S ₂	178	221	44.61	110	144	43.31
	S ₃	110	159	40.89	188	241	43.82
	S ₄	68	120	36.17	124	171	42.03
	S ₅	157	214	42.32	127	154	45.20

Back to article page

ISSN: 1480-9222

Contact us

General enquiries: journalsubmissions@springernature.com