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Table 1 Original measurements for all S k analysed by O 1 and O 2

From: Cell Counting and Viability Assessment of 2D and 3D Cell Cultures: Expected Reliability of the Trypan Blue Assay

  O 1 O 2
Live cells Dead cells Viability [%] Live cells Dead cells Viability [%]
A 1 S 1 271 39 87.42 306 33 90.27
S 2 330 51 86.61 339 41 89.21
S 3 327 37 89.84 297 28 91.38
S 4 363 24 93.80 345 23 93.75
S 5 336 40 89.36 394 30 92.92
A 2 S 1 234 92 71.78 325 77 80.85
S 2 178 57 75.74 320 71 81.84
S 3 176 48 78.57 274 53 83.79
S 4 250 67 78.86 204 55 78.76
S 5 442 102 81.25 244 50 82.99
A 3 S 1 277 114 70.84 218 79 73.40
S 2 259 108 70.57 241 87 73.48
S 3 297 111 72.79 309 101 75.37
S 4 253 76 76.90 220 182 54.73
S 5 247 86 74.17 178 64 73.55
A 4 S 1 248 84 74.70 364 137 72.65
S 2 326 121 72.93 390 136 74.14
S 3 173 53 76.55 407 133 75.37
S 4 303 105 74.26 343 119 74.24
S 5 301 106 73.96 364 122 74.90
A 5 S 1 131 119 52.40 202 145 58.21
S 2 130 113 53.50 218 227 48.99
S 3 143 64 69.08 110 24 82.09
S 4 166 64 72.17 172 49 77.83
S 5 166 83 66.67 259 68 79.20
A 6 S 1 91 12 88.35 162 88 64.80
S 2 46 35 56.79 116 76 60.42
S 3 81 33 71.05 83 40 67.48
S 4 93 49 65.49 100 48 67.57
S 5 101 50 66.89 128 60 68.09
A 7 S 1 198 206 49.01 108 103 51.18
S 2 244 267 47.75 165 126 56.70
S 3 208 163 56.06 249 190 56.72
S 4 207 130 61.42 177 146 54.80
S 5 146 120 54.89 201 174 53.60
A 8 S 1 111 181 38.01 142 200 41.52
S 2 147 294 33.33 121 220 35.48
S 3 178 179 49.86 199 220 47.49
S 4 169 137 55.23 129 142 47.60
S 5 147 118 55.47 106 128 45.30
P 1 S 1 107 11 95.24 100 5 90.68
S 2 80 8 96.25 77 3 90.91
S 3 101 9 95.18 79 4 91.82
S 4 83 7 95.59 65 3 92.22
S 5 70 6 95.65 88 4 92.11
P 2 S 1 106 17 86.87 86 13 86.18
S 2 118 21 90.00 99 11 84.89
S 3 99 12 87.60 106 15 89.19
S 4 107 12 80.00 80 20 89.92
S 5 119 14 78.50 84 23 89.47
P 3 S 1 63 14 77.61 52 15 81.82
S 2 46 14 74.14 43 15 76.67
S 3 52 10 81.69 58 13 83.87
S 4 75 17 72.73 56 21 81.52
S 5 52 11 75.86 44 14 82.53
P 4 S 1 55 48 54.17 39 33 53.40
S 2 57 44 43.48 30 39 56.44
S 3 49 44 51.04 49 47 52.69
S 4 40 30 55.65 69 55 57.14
S 5 38 42 57.43 85 63 47.50
P 5 S 1 14 116 11.59 8 61 10.77
S 2 13 91 9.26 5 49 12.50
S 3 15 127 16.22 12 62 10.56
S 4 18 138 10.26 8 70 11.54
S 5 11 71 13.33 10 65 13.41
SP 1 S 1 100 69 59.17 133 82 61.86
S 2 116 106 52.25 94 72 56.63
S 3 136 88 60.71 72 39 64.86
S 4 116 87 57.14 100 40 71.43
S 5 163 96 62.93 80 45 64.00
SP 2 S 1 155 120 56.36 66 73 47.48
S 2 125 94 57.08 125 71 63.78
S 3 158 87 64.49 103 74 58.19
S 4 154 75 67.25 85 68 55.56
S 5 156 81 65.82 219 177 55.30
SP 3 S 1 167 42 79.90 117 18 86.67
S 2 191 40 82.68 97 13 88.18
S 3 128 41 75.74 180 23 88.67
S 4 109 39 73.65 113 21 84.33
S 5 146 34 81.11 130 22 85.53
SP 4 S 1 101 71 58.72 58 33 63.74
S 2 114 65 63.69 163 61 72.77
S 3 92 60 60.53 141 45 75.81
S 4 92 53 63.45 124 60 67.39
S 5 179 77 69.92 121 56 68.36
SP 5 S 1 260 96 73.03 140 57 71.07
S 2 207 88 70.17 282 45 86.24
S 3 232 64 78.38 173 53 76.55
S 4 192 56 77.42 209 53 79.77
S 5 263 75 77.81 69 24 74.19
SP 6 S 1 222 65 77.35 175 41 81.02
S 2 226 66 77.40 229 59 79.51
S 3 216 53 80.30 108 29 78.83
S 4 218 54 80.15 135 37 78.49
S 5 205 44 82.33 254 43 85.52
SP 7 S 1 134 101 57.02 159 93 63.10
S 2 161 128 55.71 235 124 65.46
S 3 151 134 52.98 83 70 54.25
S 4 180 106 62.94 134 97 58.01
S 5 190 119 61.49 91 78 53.85
SP 8 S 1 146 197 42.57 67 105 38.95
S 2 178 221 44.61 110 144 43.31
S 3 110 159 40.89 188 241 43.82
S 4 68 120 36.17 124 171 42.03
S 5 157 214 42.32 127 154 45.20