Cell Line | Chopstick TER (\( \overset{-}{x} \) ± SEM Ω x cm2) | Endohm TER (\( \overset{-}{x} \) ± SEM Ω x cm2) |
---|
Substrate | PC | PET | PC | PET |
---|
MCF-7 (n = 16) | 402 ± 28a*
| 424 ± 12b*
| 435 ± 31a*
| 462 ± 16b*
|
MDCK (n = 5) | 7049 ± 355c**
| 5280 ± 352d**
| 8055 ± 263c**
| 6431 ± 361d**
|
MDA-MB-157 (n = 7) | 7 ± 1 | 13 ± 4 | 1 ± 0.5 | 6 ± 0.2 |
- Values represent means ± standard error of the mean. Levels of significance were tested separately for each of the cell lines using a paired t-test. a
p ≤ 0.01 when comparing MCF-7’s TER values using PC insert but different techniques. b
p ≤ 0.02 when comparing MCF-7’s TER values using PET insert but different techniques. * No significant difference between peak TER values obtained when MCF-7 ells were seeded on PC versus PET inserts using the same approach (p ≥ 0.4 for both Chopstick and Endohm). c
p = 0.04 when comparing MDCK’s TER values using PC insert but different techniques; d
p = 0.0003 MDCK’s TER values level of significance when comparing different techniques using PET insert. **Statistically significant differences when comparing MDCK’s peak TER values obtained with PC versus PET inserts using the same approach (Chopstick p = 0.009 and Endohm p = 0.002). No statistical analysis was done for MDA-MB-157 cells as they did not form functional TJs