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Table 1 Single DNA methylation from liquid biopsy as Biomarkers for lung cancer diagnosis

From: Methylated DNA/RNA in Body Fluids as Biomarkers for Lung Cancer

DNA methylation Body fluid Method Number of cases Number of controls Sensitivity (%)/specificity (%) or main findings References
SHOX2 plasma qMSP 222 189 60/90 [76]
plasma qMSP 38 31 80.65/78.57 [46]
DCLK1 plasma qMSP 65 95 49.2/91.6 [17]
SEPT9 plasma qMSP 75 100 44.3/92.3 [106]
IEAA blood HM450K 43 1986 one unit increase in IEAA was associated with 50% higher risk for LC [107]
RARβ2 plasma MSP 52 26 63/51 [50]
csbDNA MSP 52 26 70/63 [50]
DLEC1 plasma MSP 78 50 36/98 [85]
CDH1 serum qMSP 76 30 62/70 [79]
DCC serum qMSP 76 30 35.5/100 [79]
CDH13 plasma MSP 63 36 33/83 [108]
P16 serum MSP 22 0 14% [81]
plasma F-MSP 35 15 40/100 [82]
plasma modified semi-nested MSP 105 0 73% [83]
Plasma F-MSP 30 30 50% [57]
EBC F-MSP 30 30 40% [57]
DAPK serum MSP 22 0 18% [81]
serum NA 50 0 40% [80]
GSTP1 serum MSP 22 0 5% [81]
MGMT serum MSP 22 0 18% [81]
TMS1 serum NA 50 0 34% [80]
RASSFS1A serum NA 50 0 34% [80]
blood cell NA NA NA positive with LC diagnosis. [75]
APC Serum/plasma MSP 89 50 47% [84]
LINE-1 csbDNA MIRA 56 44 AUC0.69 [49]
Buffy coat DNA PCR pyrosequencing 34 360 Hypomethylation is associated with 3.2-fold higher risk for LC [51]
p53 peripheral lymphocyte DNA HpaII quantitative PCR 100 - Hypomethylation was associated with a 2-fold increased risk for LC [52]
  1. qMSP quantitative methylation-specific PCR, F-MSP fluorescent methylation-specific PCR, HM450K HumanMethylation450K BeadChip Assay, MSP methylation-specific PCR, MIRA methylated CpG island recovery assay, LC lung cancer, SHOX2 short stature homebox 2, DCLK1 doublecortin like kinase 1, SEPT9 septin9, IEAA intrinsic epigenetic age acceleration, RARβ2 retinoic acid receptor B2, DLEC1 Deleted in lung and esophageal cancer 1, CDH1 cadherin 1, DCC DCC netrin 1 receptor, CDH13 cadherin 13, DAPK death-associated protein kinase, GSTP1 glutathione S-transferase P1, MGMT O6 - methylguanine-DNA-methyltransferase, RASSF1A ras association domain family 1 isoform A, APC adenomatous polyposis coli, p16 cyclin-dependent kinase inhibitor 2A, csbDNA cell-surface-bound circulating DNA, EBC exhaled breath condensate, NA not available