Table 2 Primer lists in vector constructions
From: Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins
Primer name | Sequences | Enzyme site |
|---|---|---|
IAA1F | 5′-cccgggAATGGAAGTCACCAATGGGC-3′ | SmaI |
IAA1R | 5′-gaattcTCATAAGGCAGTAGGAGCTTCG-3′ | EcoRI |
IAA7-BF | 5′-ggatccATGATCGGCCAACTTATGAACC-3′ | BamHI |
IAA7-BR | 5′-gaattcTCAAGATCTGTTCTTGCAGTAC-3′ | EcoRI |
IAA28F | 5′-ggatccATGGAAGAAGAAAAGAGATTGG-3′ | BamHI |
IAA28R | 5′-gaattcCTATTCCTTGCCATGTTTTCTAG-3′ | EcoRI |
IAA7-SF | 5′-gtcgacATGATCGGCCAACTTATGAACCTC-3′ | SalI |
IAA7-SR | 5′-gcggccgcTCAAGATCTGTTCTTGCAGTACTTC-3′ | NotI |