Table 1 Types of ATPS with representative examples

Polymer – polymer

PEG – dextran

Chitinase

Successful partitioning of chitinase towards bottom phase

[161]

PEG – dextran

Nanospheres, nanowires and DNA derivatized nanowires

Successful In situ binding Au nanospheres with Au nanowires

[162]

Polymer – salt

PEG – K₂HPO₄

B-phycoerythin

Recovery yield = 90 %

[163]

Purification factor = 4

PEG 4000 – sulfate + 8.8 % NaCl

α-Amylase

Purification = 53 fold

[164]

Purity = 86 %

Alcohol – salt

2-propanol – K₂HPO₄

Lipase

Recovery yield = 99 % Purification factor = 13.5

[165]

Ethanol – K₂HPO₄

2,3-butanediol

Recovery yield= >98 %

[16]

Micellar/reverse micellar ATPS

n-Decyl tetra (ethylene oxide)

Bacteriophages

Bacteriophages partitioning towards micelle poor phase

[12]

Isooctane/ethylhexanol/methyltrioctyl ammoniumchloride

Plasmid DNA

Successful purification of DNA and RNA removal

[166]

Ionic liquids (ILs) – based ATPS

1-Butyl-3-methylimidazolium chloride – salt

Codeine and papaverine

Recovery yield= >90 % (codeine), >99 % (papaverine)

[167]

Imidazolium – K₂HPO₄

Curcuminoids

Extraction yield = 96 %

[168]

Purity= >51 %