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Table 1 Types of ATPS with representative examples

From: Aqueous two-phase system (ATPS): an overview and advances in its applications

Types of ATPS Representative examples Reference
Composition of ATPS Product Results
Polymer – polymer PEG – dextran Chitinase Successful partitioning of chitinase towards bottom phase [161]
PEG – dextran Nanospheres, nanowires and DNA derivatized nanowires Successful In situ binding Au nanospheres with Au nanowires [162]
Polymer – salt PEG – K2HPO4 B-phycoerythin Recovery yield = 90 % [163]
Purification factor = 4
PEG 4000 – sulfate + 8.8 % NaCl α-Amylase Purification = 53 fold [164]
Purity = 86 %
Alcohol – salt 2-propanol – K2HPO4 Lipase Recovery yield = 99 % Purification factor = 13.5 [165]
Ethanol – K2HPO4 2,3-butanediol Recovery yield= >98 % [16]
Micellar/reverse micellar ATPS n-Decyl tetra (ethylene oxide) Bacteriophages Bacteriophages partitioning towards micelle poor phase [12]
Isooctane/ethylhexanol/methyltrioctyl ammoniumchloride Plasmid DNA Successful purification of DNA and RNA removal [166]
Ionic liquids (ILs) – based ATPS 1-Butyl-3-methylimidazolium chloride – salt Codeine and papaverine Recovery yield= >90 % (codeine), >99 % (papaverine) [167]
Imidazolium – K2HPO4 Curcuminoids Extraction yield = 96 % [168]
Purity= >51 %