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Fig. 8 | Biological Procedures Online

Fig. 8

From: Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP

Fig. 8

Development of software for the automated and improved analysis of FLIP images. a-b A new method to quantify FLIP signals described in the text and in “Experimental procedures” was applied to the FLIP analysis presented in Fig. 5 (mini-FLIP). The values reported in the table (a) are graphed in the scatter plot (b) and the R2 of the best-fit trend line is reported. The range of variability (var.) is calculated from 2 measurements of the fluorescence of the input lysate used for FLIP (X axis) and from 2 separate measurements of the FLIP signal from the beads (Y axis). c-d The improved FLIP analysis was applied to the screening presented in Fig. 6. The % of total lysate immunoprecipitation index (% tot. lys) calculated after IP/IB, and the improved FLIP signal, are reported in the column graph and in the table. The only supernatant (NY1.1.1D4) showing a discrepancy between IP/IB and FLIP is highlighted in gray

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