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Fig. 7 | Biological Procedures Online

Fig. 7

From: Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP

Fig. 7

FLIP applied to hybridoma supernatants. 10 hybridoma supernatants produced after immunization with a UPF1 polypeptide were tested by FLIP and IP/IB. a The results of IP/IB, direct examination of the pictures collected after FLIP and ImageJ analysis of the FLIP results are reported in the table. b One of the two pictures collected after FLIP is also shown for each of the supernatants, FLAG and IgG IP. c IP/IB analysis of the 10 supernatants tested. Immunoblotting was performed using a FLAG antibody and tubulin was used as loading control

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