Fig. 3
From: Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP
Comparison of FLIP and IP/IB sensitivity. a Correlation between amount of YFP used and FLIP signal. Two FLIP measurements from 2 different aliquots of beads were used to calculate the range of variability of the measurement (var.). b Immunoblot analysis of the same samples used in (a) A FLAG antibody was used as a positive control for IP and Western detection. Tubulin was used as a loading control for the total lysate used for IP and for the lysate of untransfected cells used as negative