Fig. 5From: An Optimized Protocol for Packaging Pseudotyped Integrase Defective LentivirusTransgene expression in nondividing cells. Mouse embryonic fibroblasts (STO) were treated with actinomycin to make nondividing cells. The treated cells were subsequently transduced with IDLV reporter viruses. Images were taken using either GFP-filter (left panel), RFP-filter (middle panel) or phase contrast (right panel) to show the reporter expressionBack to article page