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Table 1 Troubleshooting

From: Reliable FASP-based procedures for optimal quantitative proteomic and phosphoproteomic analysis on samples from acute myeloid leukemia patients

Problem

Possible reason

Solution

The centrifugal unit spun all the protein sample. No sample left on the membrane.

Faulty filter

Collect the filtrate and place it onto a new tested and conditioned spin filter

Filter membrane displaced due to the tight insertion of the filter into the collection tube

Find a collection tube where a new tested and conditioned filter can be inserted with no friction. Collect the filtrate onto it.

Low peptide recovery in the filtrate (<40 %)

Severe filter clogging

Pipette gently up and down to homogenize when adding a new solution, without touching the filter membrane