Fig. 1

Description of the pBBknock vector, knockout experimental design and protocol. a Vector description; all the elements are described in the box below the panel. b The AB DNA sequence is assembled in the pBBknock vector and the resulting plasmid is used to carry out chromosomal gene deletion via two successive recombination events, described in panel c. After the two recombination events, the resulting genomic target sequence is shown: it has about 50 % probability to be successfully modified or to revert to the wild type state (not shown). c Allele replacement protocol description. Notes on protocol development are reported in Additional file 1: Notes on protocol development