Table 2 Method settings in ChemStation for system calibration

Pump Parameter

 Flow

0.5 mL/min

 Stop Time

60 min

 Solvent A

100 %

 Pressure Limits Max

system pressure + 18 MPa

Injector

 Standard Injection

Enable

 Injection Volume

100 μL

DAD Parameter (UV diode array)

 Signals Store

Enable 280 nm

 Stop Time

As pump

 Peakwidth

1 s

 Slit

4 nm