Fig. 5

SEC as monitored by UV absorbance, refractive index, and light scattering of Mistic solubilised in DDM or NM and of protein-free DDM micelles in the presence of 6 M urea. UV absorbance and RI values as well as the voltage of the LS detector are depicted as functions of elution volume. a 6.08 mM DDM without protein gives rise to a single peak at ~9.3 mL in both RI and LS signals but shows no UV absorbance. b Mistic solubilised in 29.6 mM NM produces two peaks, namely, one at 9.3 mL representing unfolded protein and a second one at ~13 mL reflecting protein-free detergent micelles. c Mistic solubilised in 6.08 mM DDM reveals only a single peak at ~9.3 mL in all three detectors. 1 mg/mL Mistic, 50 mM Tris, pH 7.4, 50 mM NaCl, c _det,sample = CMC_{8 M urea} + 5 mM, c _det,buffer = CMC_buffer + 2 mM, flow rate 0.4 mL/min, RT