Fig. 1
From: Characterising protein/detergent complexes by triple-detection size-exclusion chromatography
Schematic setup of triple-detection SEC. A high-performance liquid chromatography (HPLC) system is used to provide constant flow and an SEC column to separate different species. UV absorbance and RI detectors monitor changes in protein and detergent concentrations, while an LS detector follows changes in scattering intensity at multiple scattering angles. Data acquisition is controlled by a software package