Fig. 4

Comparing segmentation results on raw STED image, with and without 3x3 smooth or band pass filters. a, b Segmentation image of gp210 donuts (a) and gp210 clusters (b). c Number of donuts segmented from raw image, smoothed image, and band pass filtered image. d Over segmentation artifacts of gp210 clusters per gp210 donuts significantly reduced in image pre-processed by a bandpass filter using ImageJ