Fig. 2
From: CellProfiler: Novel Automated Image Segmentation Procedure for Super-Resolution Microscopy
Automated counting of gp210 and pan-FG protein clusters per nuclear pore complex. a Overview showing pipeline modules sequence. b Influence of Gaussian blurring on structure of gp210 STED resolved structures. c Two color raw STED image of immunostained gp210 (red) and pan-FG (green) and images series produced after segmentation. d Zoom in image of segmentation results from CellProfiler window showing two neighbouring nuclear pores (left), gp210 clusters (middle), and pan-FG clusters (right). e Histogram plots showing quantitative segmentation results. f Current model of nuclear pore complex and two direction arrows connecting models with experimental results