Table 3 qPCR reaction components
From: Detection and quantification of extracellular microRNAs in murine biofluids
Component | μl per reaction (1X) |
|---|---|
TaqMan small RNA assay (20X) | 1 |
Product from RT reaction/standard | 1.33 |
TaqMan mastermix | 10 |
Nuclease-free water | 7.67 |
(Total) | (20) |