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Table 1 RT reaction components

From: Detection and quantification of extracellular microRNAs in murine biofluids

Component μl per reaction (1X)
10X miRNA RT buffer 2
MultiScribe RT (50 U/μl) 1
100 mM dNTP mix 0.15
RNase Inhibitor (20 U/μl) 0.18
20X miRNA RT primer - assay A 1
20X miRNA RT primer - assay B 1
20X miRNA RT primer - assay C 1
20X miRNA RT primer - assay D 1
Water 7.67
(Total) (15)