Figure 6
From: Detection and quantification of extracellular microRNAs in murine biofluids
Determination of PCR efficiency using LinRegPCR. Screenshot of LinRegPCR software showing sample data with key feature highlighted. (a) Amplification plot (i.e. Log(Fluorescence) vs Cycle). (b) Window-of-linearity (included points are shown in red). (c) PCR efficiency and correlation coefficient (R) indicating how well the points fit the analysed linear region for a single reaction. (d) PCR efficiencies and correlation coefficients for all samples. (e) Two samples (the no template controls) failed to amplify and have PCR efficiencies ~1 (i.e. 0%).